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培養基配方

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YM-1L Broth

[所屬分類:培養基配方] [發布時間:2021-12-29] [發布人:網站管理員2] [閱讀次數:] [返回]

YM-1L Broth

山東拓普生物工程有限公司培養基配方 http://www.apro-anicetech.com

Composition per liter:

Sodium lactate ..................................................30.0g

Mycological peptone ........................................10.0g

Succinic acid.....................................................10.0g

NaOH..................................................................6.0g

Yeast extract........................................................5.0g

Adenine.............................................................0.01g

Uracil ................................................................0.01g

Yeast nitrogen base solution ....................... 100.0mL

Yeast Nitrogen Base Solution:

Composition per 100mL:

(NH4)2SO4...........................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O.........................................................0.1g

DL-Methionine...................................................0.02g

DL-Tryptophan...................................................0.02g

L-Histidine·HCl .................................................0.01g

Inositol .............................................................2.0mg

KI .....................................................................0.1mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O ...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyroxidine·HCl ................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

CuSO4·5H2O ..................................................0.04mg

Folic acid.......................................................... 2.0μg

Biotin ............................................................... 2.0μg

Preparation of Yeast Nitrogen Base Solution:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Filter sterilize

Preparation of Medium: Add components, ex

cept yeast nitrogen base solution, to distilled/deion

ized water and bring volume to 900.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 1.0L of sterile yeast nitrogen

base solution. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of Saccharomyces cerevisiae.

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YM-1L Broth