培養基配方

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Z-Agar

[所屬分類:培養基配方] [發布時間:2021-12-30] [發布人:網站管理員2] [閱讀次數:] [返回]

Z-Agar

山東拓普生物工程有限公司培養基配方 http://www.apro-anicetech.com

Composition per liter:

Agar ........................................................... 16.0

K2HPO4...............................................................5.0g

K2SO4..................................................................2.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ....................................................0.05g

Acetamide solution ....................................... 40.0mL

pH 7.2 ± 0.2 at 25°C

Acetamide Solution:

Composition per 100.0mL:

Acetamide .........................................................10.0g

Preparation of Acetamide Solution: Add aceta

mide to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept acetamide solution, to distilled/deionized water

and bring volume to 960.0mL. Mix thoroughly. Au

toclave for 20 min at 15 psi pressure–121°C. Cool to

70°C. Aseptically add 40.0mL sterile acetamide so

lution. Mix thoroughly. Pour into sterile Petri dishes.

Dry plates at 37°C for 30 min.

Use: For the isolation of Pseudomonas aeruginosa

from milk.